pQE9-His-p97deltaD2
(Plasmid
#17229)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQE9
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Backbone manufacturerQiagen
- Backbone size w/o insert (bp) 3300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Growth instructionsXL1 Blue, need stain with lacIq
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namep97
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Alt namevalosin-containing protein
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SpeciesM. musculus (mouse)
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Insert Size (bp)2400
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Mutationchanged codon 457 to stop codon
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Entrez GeneVcp (a.k.a. 3110001E05, CDC48, p97, p97/VCP)
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQE9-His-p97deltaD2 was a gift from Hemmo Meyer (Addgene plasmid # 17229 ; http://n2t.net/addgene:17229 ; RRID:Addgene_17229) -
For your References section:
Cdc48/p97 promotes reformation of the nucleus by extracting the kinase Aurora B from chromatin. Ramadan K, Bruderer R, Spiga FM, Popp O, Baur T, Gotta M, Meyer HH. Nature. 2007 Dec 20. 450(7173):1258-62. 10.1038/nature06388 PubMed 18097415