pcDNA3.1 puro Nodamura B2
(Plasmid #17228)

• Depositing Lab: Christopher Sullivan
• Publication: Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Backbone

• Vector backbone: pcDNA3.1 puro+
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nodamura B2
• Species: Nodamura Virus
• Insert Size (bp): 429
• Mutation: 2 silent point mutations
• Entrez Gene: NoVsRNA1gp2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Plasmid map
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

The gene was cloned into the KpnI/XbaI sites within the pcDNA3.1 puro+ vector. The pcDNA3.1 puro+ vector was derived from the pcDNA3.1 neo+ vector by swapping the puro cassette for the Neo gene.