pMIG-hKLF4
(Plasmid #17227)

• Depositing Lab: George Daley
• Publication: Park et al Nature. 2008 Jan 10. 451(7175):141-6.

Backbone

• Vector backbone: pMIG
• Backbone size w/o insert (bp): 6500
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Kruppel-like factor 4 (gut)
• Alt name: KLF4
• Species: H. sapiens (human)
• Insert Size (bp): 1413
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pLXSN 5'

Resource Information

• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The hKlf4 in this plasmid matches GenBank's protein AAH30811.1, and also has an S240G amino acid change.

Addgene has confirmed the presence of GFP in the pMIG vector backbone by sequencing, but the functionality of the GFP has not been confirmed.

Note that the pMIG-cMyc used in this paper can be found here: www.addgene.org/18119 .