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pLenti6/UbC/mSlc7a1
(Plasmid #17224)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17224 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6/UbC/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7600
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    We recommend using Stbl3 E. coli strain (Invitrogen).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Slc7a1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1870
  • Entrez Gene
    Slc7a1 (a.k.a. 4831426K01Rik, AI447493, Atrc-1, Atrc1, Cat1, Rec-1, Rev-1, mCAT-1)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attB1 (not destroyed)
  • 3′ cloning site attB2 (not destroyed)
  • 5′ sequencing primer hUBCpro-F (5'-TGAAGCTCCGGTTTTGAACT-3')
  • 3′ sequencing primer pBABE3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6/UbC/mSlc7a1 was a gift from Shinya Yamanaka (Addgene plasmid # 17224 ; http://n2t.net/addgene:17224 ; RRID:Addgene_17224)

  • For your References section:

    Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Cell. 2007 Nov 30. 131(5):861-72. 10.1016/j.cell.2007.11.019 PubMed 18035408
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