mCherry-2A-eGFP(W58X) pcDNA3.1(-)
(Plasmid
#172229)
-
PurposeExpresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3.1(-)
- Total vector size (bp) 6898
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemCherry-2A-eGFP
-
Insert Size (bp)1514
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
mCherry-2A-eGFP(W58X) pcDNA3.1(-) was a gift from Joshua Rosenthal (Addgene plasmid # 172229 ; http://n2t.net/addgene:172229 ; RRID:Addgene_172229) -
For your References section:
An efficient system for selectively altering genetic information within mRNAs. Montiel-Gonzalez MF, Vallecillo-Viejo IC, Rosenthal JJ. Nucleic Acids Res. 2016 Dec 1;44(21):e157. doi: 10.1093/nar/gkw738. Epub 2016 Aug 23. 10.1093/nar/gkw738 PubMed 27557710
Map uploaded by the depositor.
