4LNDDE488Q-pCDNA3.1(+)
(Plasmid #172228)

• Purpose: Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells
• Depositing Lab: Joshua Rosenthal
• Publication: Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e157. doi: 10.1093/nar/gkw738. Epub 2016 Aug 23.

Backbone

• Vector backbone: pcDNA3.1(+)
• Total vector size (bp): 7085
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lambda N peptide - human ADAR2 Deaminase Domain E488Q
• Species: H. sapiens (human); Escherichia virus Lambda
• Insert Size (bp): 1661
• Mutation: changed the Glutamic acid 488 in the Deaminase Domain for Glutamine
• Promoter: CMV
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGHrev

Resource Information

• Supplemental Documents:
  • 4lNDD_E488Q_pcDNA3.1(+).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry