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Addgene

4LNDD-pCDNA3.1(+)
(Plasmid #172227)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172227 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Total vector size (bp) 7085
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lambda N peptide - human ADAR2 Deaminase Domain
  • Species
    H. sapiens (human); Escherichia virus Lambda
  • Insert Size (bp)
    1661
  • GenBank ID
    NG_052015.1 NC_001416.1
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4LNDD-pCDNA3.1(+) was a gift from Joshua Rosenthal (Addgene plasmid # 172227 ; http://n2t.net/addgene:172227 ; RRID:Addgene_172227)

  • For your References section:

    An efficient system for selectively altering genetic information within mRNAs. Montiel-Gonzalez MF, Vallecillo-Viejo IC, Rosenthal JJ. Nucleic Acids Res. 2016 Dec 1;44(21):e157. doi: 10.1093/nar/gkw738. Epub 2016 Aug 23. 10.1093/nar/gkw738 PubMed 27557710
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