PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA
(Plasmid #172226)

• Purpose: Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.
• Depositing Lab: Alexander Meissner
• Publication: Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi: 10.1038/s41467-021-24951-7.

Backbone

• Vector backbone: PB-CAG-BGHpA (Plasmid #92161)
• Backbone manufacturer: Xiaohua Shen
• Backbone size w/o insert (bp): 7531
• Total vector size (bp): 10010
• Vector type: Mammalian Expression ; piggyBAC transposon
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SOX17
• Species: H. sapiens (human)
• Insert Size (bp): 2572
• GenBank ID: NM_022454.4
• Entrez Gene: SOX17 (a.k.a. VUR3)
• Promoter: CAG
• Tags / Fusion Proteins:
  • DD-domain (N terminal on insert)
  • 3XFLAG (N terminal on insert)
  • GS-linker (C terminal on insert)
  • TagBFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n.a.
• 3′ sequencing primer: TAGAAGGCACAGTCGAGG

Resource Information

• Supplemental Documents:
  • PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA.gb
• A portion of this plasmid was derived from a plasmid made by: The DD-3xFLAG-hSOX17-GS-TagBFP expression cassette was originally synthesized and cloned into pUC19 by Genewiz. The cassette was extracted from pUC19 via SapI and further inserted into BstBI/BamHI digested PB-CAG-BGHpA.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry