pCjedCas9
(Plasmid #172216)

• Purpose: Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells
• Depositing Lab: John van der Oost
• Publication: Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi: 10.1126/sciadv.aaz4849. eCollection 2020 Jun.

Backbone

• Vector backbone: His6 TEV LIC cloning vector (1B)
• Backbone size w/o insert (bp): 5353
• Total vector size (bp): 8302

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
• Alt name: CjeCas9
• Species: Synthetic
• Insert Size (bp): 2949
• Mutation: changed Asp 8 to Ala and His 559 to Ala
• Tag / Fusion Protein:
  • Histag

Resource Information

• Supplemental Documents:
  • pML1B-CjdCas9 (catalytic mutant).gbk
  • pML1B-CjdCas9 (catalytic mutant).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry