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Addgene

pCjeCas9
(Plasmid #172215)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172215 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    His6 TEV LIC cloning vector (1B)
  • Backbone size w/o insert (bp) 5353
  • Total vector size (bp) 8302

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
  • Alt name
    CjeCas9
  • Species
    Synthetic
  • Insert Size (bp)
    2949
  • Promoter T7
  • Tag / Fusion Protein
    • Histag

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCjeCas9 was a gift from John van der Oost (Addgene plasmid # 172215 ; http://n2t.net/addgene:172215 ; RRID:Addgene_172215)

  • For your References section:

    Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA. Saha C, Mohanraju P, Stubbs A, Dugar G, Hoogstrate Y, Kremers GJ, van Cappellen WA, Horst-Kreft D, Laffeber C, Lebbink JHG, Bruens S, Gaskin D, Beerens D, Klunder M, Joosten R, Demmers JAA, van Gent D, Mouton JW, van der Spek PJ, van der Oost J, van Baarlen P, Louwen R. Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi: 10.1126/sciadv.aaz4849. eCollection 2020 Jun. 10.1126/sciadv.aaz4849 PubMed 32596446
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