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Addgene

pEx-insertion-tet
(Plasmid #172211)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172211 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pExchange-tdk
  • Backbone manufacturer
    Koropatkin, N.M. (PMID: 18611383)
  • Backbone size (bp) 4172
  • Vector type
    Genetic insertion in Prevotella copri
  • Selectable markers
    Tetracycline in Prevotella copri

Growth in Bacteria

  • Bacterial Resistance(s)
    Amp + DAP
  • Growth Temperature
    37°C
  • Growth Strain(s)
    β2155
  • Growth instructions
    Supplemented Media with 0.3 mM 2,6-Diaminopimelic acid (DAP)
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEx-insertion-tet was a gift from Till Strowig (Addgene plasmid # 172211 ; http://n2t.net/addgene:172211 ; RRID:Addgene_172211)

  • For your References section:

    A versatile genetic toolbox for Prevotella copri enables studying polysaccharide utilization systems. Li J, Galvez EJC, Amend L, Almasi E, Iljazovic A, Lesker TR, Bielecka AA, Schorr EM, Strowig T. EMBO J. 2021 Dec 1;40(23):e108287. doi: 10.15252/embj.2021108287. Epub 2021 Oct 21. 10.15252/embj.2021108287 PubMed 34676563
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