eGFP-KIF1A-Rigor
(Plasmid #172207)

• Purpose: Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells
• Depositing Lab: Juan Bonifacino
• Publication: Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. doi: 10.1016/j.celrep.2016.10.062.

Backbone

• Vector backbone: eGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 9798
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KIF1A
• Species: M. musculus (mouse)
• Insert Size (bp): 2889
• Mutation: Changed Glycine 251 to Alanine
• Entrez Gene: Kif1a (a.k.a. A, ATSV, C630002N23Rik, Gm1626, Kn, Kns1)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: SV40_rev

Resource Information

• Supplemental Documents:
  • eGFP-mKIF1A-Rigor.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry