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pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL
(Plasmid #172205)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV_hSyn_WPRE_bGHpA
  • Backbone manufacturer
    Zhang Lab
  • Backbone size w/o insert (bp) 2884
  • Total vector size (bp) 7335
  • Modifications to backbone
    Replaced the WPRE_bGHpA cassette with the modified W3SL cassette (Choi et al Mol Brain. 2014) to maximize insert size and remain within AAV packaging limits
  • Vector type
    Mammalian Expression, Mouse Targeting, AAV ; TALE

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
  • Alt name
    Tet1FL-SID4X
  • Species
    Synthetic
  • Insert Size (bp)
    4451
  • Promoter hSyn human synapsin (mammalian neuronal expression)
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • T2A-EGFP (C terminal on insert)
    • NLS

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer hSyn_promoter_seqF 5'-CCTCAGCACTGAAGGC-3'
  • 3′ sequencing primer W3SL_seqR 5'-CGTATGCGGTGTGAAATA-3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL backbone vector was generated via Gibson Assembly using PCR fragments amplified from pAAV_CW3SL-EGFP, a gift from Bong-Kiun Kaang (Addgene plasmid # 61463) as well as pAAV_hSyn_TALEBB(NG)-NLS-VP64_2A_GFP_WPRE_bGHpA and pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA, gifts from Feng Zhang (Addgene plasmids # 47447, # 47451, respectively).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL was a gift from Garrett Kaas (Addgene plasmid # 172205 ; http://n2t.net/addgene:172205 ; RRID:Addgene_172205)

  • For your References section:

    Tet1 Isoforms Differentially Regulate Gene Expression, Synaptic Transmission, and Memory in the Mammalian Brain. Greer CB, Wright J, Weiss JD, Lazarenko RM, Moran SP, Zhu J, Chronister KS, Jin AY, Kennedy AJ, Sweatt JD, Kaas GA. J Neurosci. 2021 Jan 27;41(4):578-593. doi: 10.1523/JNEUROSCI.1821-20.2020. Epub 2020 Dec 1. 10.1523/JNEUROSCI.1821-20.2020 PubMed 33262245
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