eGFP-KIF5B-Rigor
(Plasmid
#172204)
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PurposeExpresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneeGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4731
- Total vector size (bp) 7620
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKIF5B
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SpeciesM. musculus (mouse)
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Insert Size (bp)2889
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Entrez GeneKif5b (a.k.a. Khc, Khcs, Kns1, Ukhc)
- Promoter CMV
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_fwd
- 3′ sequencing primer SV40_rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
eGFP-KIF5B-Rigor was a gift from Juan Bonifacino (Addgene plasmid # 172204 ; http://n2t.net/addgene:172204 ; RRID:Addgene_172204) -
For your References section:
BORC Functions Upstream of Kinesins 1 and 3 to Coordinate Regional Movement of Lysosomes along Different Microtubule Tracks. Guardia CM, Farias GG, Jia R, Pu J, Bonifacino JS. Cell Rep. 2016 Nov 15;17(8):1950-1961. doi: 10.1016/j.celrep.2016.10.062. 10.1016/j.celrep.2016.10.062 PubMed 27851960