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eGFP-KIF5A-Rigor
(Plasmid #172202)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172202 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    eGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4728
  • Total vector size (bp) 7809
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KIF5A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3081
  • Mutation
    Changed Glycine 235 to Alanine
  • Entrez Gene
    Kif5a (a.k.a. D10Bwg0738e, Khc, Kif5, Kns, mKIAA4086)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eGFP-KIF5A-Rigor was a gift from Juan Bonifacino (Addgene plasmid # 172202 ; http://n2t.net/addgene:172202 ; RRID:Addgene_172202)

  • For your References section:

    Sorting of Dendritic and Axonal Vesicles at the Pre-axonal Exclusion Zone. Farias GG, Guardia CM, Britt DJ, Guo X, Bonifacino JS. Cell Rep. 2015 Nov 10;13(6):1221-1232. doi: 10.1016/j.celrep.2015.09.074. Epub 2015 Oct 29. 10.1016/j.celrep.2015.09.074 PubMed 26527003
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