eGFP-KIF5A
(Plasmid
#172201)
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PurposeExpresses KIF5A tagged with eGFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172201 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneeGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4728
- Total vector size (bp) 7809
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKIF5A
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SpeciesM. musculus (mouse)
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Insert Size (bp)3081
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Entrez GeneKif5a (a.k.a. D10Bwg0738e, Khc, Kif5, Kns, mKIAA4086)
- Promoter CMV
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_fwd
- 3′ sequencing primer SV40_rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
eGFP-KIF5A was a gift from Juan Bonifacino (Addgene plasmid # 172201 ; http://n2t.net/addgene:172201 ; RRID:Addgene_172201) -
For your References section:
Sorting of Dendritic and Axonal Vesicles at the Pre-axonal Exclusion Zone. Farias GG, Guardia CM, Britt DJ, Guo X, Bonifacino JS. Cell Rep. 2015 Nov 10;13(6):1221-1232. doi: 10.1016/j.celrep.2015.09.074. Epub 2015 Oct 29. 10.1016/j.celrep.2015.09.074 PubMed 26527003