RepX_pET28a(+)
(Plasmid #172199)

• Purpose: Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.
• Depositing Lab: Taekjip Ha
• Publication: Arslan et al Science. 2015 Apr 17;348(6232):344-7. doi: 10.1126/science.aaa0445.

Backbone

• Vector backbone: pET28a(+)
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): BL21(DE3)
• Growth instructions: Use BL21(DE3) cell line for the protein overexpression
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RepX superhelicase
• Species: E. coli
• Insert Size (bp): 2070
• Mutation: C18L, C43S, C167V, S400C, C612A
• Tag / Fusion Protein:
  • 6xHis tag (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Sinan Arslan, a former graduate student in Taekjip Ha lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see https://pubmed.ncbi.nlm.nih.gov/33657402/ for additional information