pEA02
(Plasmid #172193)

• Purpose: Suicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.
• Depositing Lab: Jean-Luc Pernodet
• Publication: Santos et al Microorganisms. 2022 Apr 16;10(4). pii: microorganisms10040828. doi: 10.3390/microorganisms10040828.

Backbone

• Vector backbone: pOJ260
• Backbone manufacturer: Bierman M. et al. Gene 1992 Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.
• Backbone size w/o insert (bp): 3469
• Total vector size (bp): 3665
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Apramycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: attL
• Alt name: Left attachment site of pSAM2 (minimal attL site as defined in Raynal et al. 1998)
• Species: Synthetic
• Insert Size (bp): 220

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CGGGCCTCTTCGCTATTAC

Resource Information

• Supplemental Documents:
  • pEA02.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry