pCS2-hFAM 3B-His
(Plasmid
#172192)
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PurposeCoding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned medium
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172192 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
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Backbone manufacturerpromega
- Backbone size w/o insert (bp) 4096
- Total vector size (bp) 4096
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehFAM 3B
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Alt namePANDER
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SpeciesH. sapiens (human)
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Insert Size (bp)542
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GenBank IDNM_058186
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-hFAM 3B-His was a gift from Edward De Robertis (Addgene plasmid # 172192 ; http://n2t.net/addgene:172192 ; RRID:Addgene_172192) -
For your References section:
The cytokine FAM3B/PANDER is an FGFR ligand that promotes posterior development in Xenopus. Zhang F, Zhu X, Wang P, He Q, Huang H, Zheng T, Li Y, Jia H, Xu L, Zhao H, Colozza G, Tao Q, De Robertis EM, Ding Y. Proc Natl Acad Sci U S A. 2021 May 18;118(20). pii: 2100342118. doi: 10.1073/pnas.2100342118. 10.1073/pnas.2100342118 PubMed 33975953