pMXs-hOCT3/4
(Plasmid #17217)

• Purpose: Retroviral expression of human OCT3/4
• Depositing Lab: Shinya Yamanaka
• Publication: Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Backbone

• Vector backbone: pMXs
• Backbone size w/o insert (bp): 4600
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OCT3/4
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: None
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pMX-S1811

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
• Articles Citing this Plasmid:
  • 108 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.