pJH291
(Plasmid
#172153)
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PurposeReporter plasmid for pylBCD variant in pCDF backbone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172153 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep15A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePara sfGFP.2TAG; Plpp pylS; PproK pylT; AraC
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SpeciesSynthetic
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MutationsfGFP(N39*, Y151*)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJH291 was a gift from Matthew Bennett (Addgene plasmid # 172153 ; http://n2t.net/addgene:172153 ; RRID:Addgene_172153) -
For your References section:
Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway. Ho JML, Miller CA, Smith KA, Mattia JR, Bennett MR. Nat Commun. 2021 Jun 24;12(1):3914. doi: 10.1038/s41467-021-24183-9. 10.1038/s41467-021-24183-9 PubMed 34168131