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Addgene

pJH77
(Plasmid #172145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172145 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ppsp gIII.3TAG, luxAB; Plpp pylS; PproK pylT
  • Species
    Synthetic
  • Mutation
    gIII(P29*, P83*, Y184*)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH77 was a gift from Matthew Bennett (Addgene plasmid # 172145 ; http://n2t.net/addgene:172145 ; RRID:Addgene_172145)

  • For your References section:

    Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway. Ho JML, Miller CA, Smith KA, Mattia JR, Bennett MR. Nat Commun. 2021 Jun 24;12(1):3914. doi: 10.1038/s41467-021-24183-9. 10.1038/s41467-021-24183-9 PubMed 34168131
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