pBad-UXPS
(Plasmid
#172138)
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Purposebacteria expression plasmid for a UDP/UTP sensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172138 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBad
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUXPS
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Alt namean engineered sensor for UTP/UDP, used as a control for UGAcS
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SpeciesSynthetic
- Promoter araBAD
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBad-F
- 3′ sequencing primer pBad-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBad-UXPS was a gift from Huiwang Ai (Addgene plasmid # 172138 ; http://n2t.net/addgene:172138 ; RRID:Addgene_172138) -
For your References section:
Genetically Encoded Green Fluorescent Biosensors for Monitoring UDP-GlcNAc in Live Cells. Li Z, Zhang J, Ai HW. ACS Cent Sci. 2021 Oct 27;7(10):1763-1770. doi: 10.1021/acscentsci.1c00745. Epub 2021 Sep 30. 10.1021/acscentsci.1c00745 PubMed 34729420