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Addgene

pBad-UGAcS
(Plasmid #172137)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172137 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBad
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    UGAcS
  • Alt name
    an engineered sensor for UDP-GlcNAc
  • Species
    Synthetic
  • Insert Size (bp)
    1818
  • Mutation
    H605Q- please see depositor comments
  • Promoter araBAD

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Due to its cross-reactivity with UDP and UTP, the UXPS sensor is a recommended control. Depositor confirms H605Q mutation in insert does not confirm function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBad-UGAcS was a gift from Huiwang Ai (Addgene plasmid # 172137 ; http://n2t.net/addgene:172137 ; RRID:Addgene_172137)

  • For your References section:

    Genetically Encoded Green Fluorescent Biosensors for Monitoring UDP-GlcNAc in Live Cells. Li Z, Zhang J, Ai HW. ACS Cent Sci. 2021 Oct 27;7(10):1763-1770. doi: 10.1021/acscentsci.1c00745. Epub 2021 Sep 30. 10.1021/acscentsci.1c00745 PubMed 34729420
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