pcDNA3-UGAcS
(Plasmid
#172135)
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Purposemammalian expression plasmid for a UDP-GlcNAc sensor
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUGAcS
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SpeciesSynthetic
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Insert Size (bp)1818
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Due to its cross-reactivity with UDP and UTP, the UXPS sensor is a recommended control.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-UGAcS was a gift from Huiwang Ai (Addgene plasmid # 172135 ; http://n2t.net/addgene:172135 ; RRID:Addgene_172135) -
For your References section:
Genetically Encoded Green Fluorescent Biosensors for Monitoring UDP-GlcNAc in Live Cells. Li Z, Zhang J, Ai HW. ACS Cent Sci. 2021 Oct 27;7(10):1763-1770. doi: 10.1021/acscentsci.1c00745. Epub 2021 Sep 30. 10.1021/acscentsci.1c00745 PubMed 34729420
