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pAAVS1_Puro_ccdb_Ubc_rtTA
(Plasmid #172130)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172130 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1_Puro_PGK1_3xFLAG_Twin_Strep (Plasmid #68375)
  • Backbone size (bp) 5967
  • Vector type
    Mammalian Expression
  • Promoter PGK1
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVS1_Puro_ccdb_Ubc_rtTA was a gift from Kenji Kontani (Addgene plasmid # 172130 ; http://n2t.net/addgene:172130 ; RRID:Addgene_172130)

  • For your References section:

    Development of a versatile HPLC-based method to evaluate the activation status of small GTPases. Araki M, Yoshimoto K, Ohta M, Katada T, Kontani K. J Biol Chem. 2021 Nov 18:101428. doi: 10.1016/j.jbc.2021.101428. 10.1016/j.jbc.2021.101428 PubMed 34801548
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