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Addgene

pLenti-pHluorin_M153R-CD63-mScarlet
(Plasmid #172118)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172118 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-CMV Blast DEST (706-1)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CD63
  • Species
    H. sapiens (human)
  • GenBank ID
    CR542096.1
  • Entrez Gene
    CD63 (a.k.a. AD1, HOP-26, ME491, MLA1, OMA81H, Pltgp40, TSPAN30)
  • Tags / Fusion Proteins
    • pHluorin_M153R in a small extracelluar loop of CD63
    • mScarlet (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CACCATGGCGGTGGAAGG
  • 3′ sequencing primer CTACATCACCTCGTAGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-pHluorin_M153R-CD63-mScarlet was a gift from Alissa Weaver (Addgene plasmid # 172118 ; http://n2t.net/addgene:172118 ; RRID:Addgene_172118)

  • For your References section:

    A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells. Sung BH, von Lersner A, Guerrero J, Krystofiak ES, Inman D, Pelletier R, Zijlstra A, Ponik SM, Weaver AM. Nat Commun. 2020 Apr 29;11(1):2092. doi: 10.1038/s41467-020-15747-2. 10.1038/s41467-020-15747-2 PubMed 32350252
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