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pLenti-pHluorin_M153R-CD63
(Plasmid #172117)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172117 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-CMV Blast DEST (706-1)
  • Backbone size w/o insert (bp) 9331
  • Total vector size (bp) 9140
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CD63
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1428
  • GenBank ID
    CR542096.1
  • Entrez Gene
    CD63 (a.k.a. AD1, HOP-26, ME491, MLA1, OMA81H, Pltgp40, TSPAN30)
  • Promoter CMV
  • Tag / Fusion Protein
    • pHluorin_M153R in a small extracelluar loop of CD63

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CACCATGGCGGTGGAAGG
  • 3′ sequencing primer CTACATCACCTCGTAGCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-pHluorin_M153R-CD63 was a gift from Alissa Weaver (Addgene plasmid # 172117 ; http://n2t.net/addgene:172117 ; RRID:Addgene_172117)

  • For your References section:

    A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells. Sung BH, von Lersner A, Guerrero J, Krystofiak ES, Inman D, Pelletier R, Zijlstra A, Ponik SM, Weaver AM. Nat Commun. 2020 Apr 29;11(1):2092. doi: 10.1038/s41467-020-15747-2. 10.1038/s41467-020-15747-2 PubMed 32350252
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