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NmMetNI-WT
(Plasmid #172114)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172114 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5245
  • Total vector size (bp) 6916
  • Modifications to backbone
    Added 4 more His to make a total of 10X His. Added Enterokinase after His tag. Removed original pelB sequence from vector backbone.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    MetN
  • Species
    N.Neisseria meningitidis serogroup B (strain MC58)
  • Insert Size (bp)
    738
  • Promoter T7 Promoter
  • Tags / Fusion Proteins
    • 10x His Tag (N terminal on insert)
    • Enterokinase (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer MetN Custom Gene Specific Primer
  • 3′ sequencing primer MetN Custom Gene Specific Primer
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    MetI
  • Species
    N.Neisseria meningitidis serogroup B (strain MC58)
  • Insert Size (bp)
    687
  • Promoter T7 Promoter

Cloning Information for Gene/Insert 2

  • Cloning method Unknown
  • 5′ sequencing primer MetI Custom Gene Specific Primer
  • 3′ sequencing primer MetI Custom Gene Specific Primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NmMetNI-WT was a gift from Douglas Rees (Addgene plasmid # 172114 ; http://n2t.net/addgene:172114 ; RRID:Addgene_172114)

  • For your References section:

    Characterization of the ABC methionine transporter from Neisseria meningitidis reveals that lipidated MetQ is required for interaction. Sharaf NG, Shahgholi M, Kim E, Lai JY, VanderVelde DG, Lee AT, Rees DC. Elife. 2021 Aug 19;10. pii: 69742. doi: 10.7554/eLife.69742. 10.7554/eLife.69742 PubMed 34409939
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