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Addgene

NmMetQ C20A
(Plasmid #172112)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172112 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5511
  • Total vector size (bp) 6246
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use of autoinduction method
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Methionine Binding Protein
  • Alt name
    MetQ
  • Species
    N.Neisseria meningitidis serogroup B (strain MC58)
  • Insert Size (bp)
    735
  • Mutation
    Changed Proline 22 to Alanine on MetQ and Cysteine 20 to Alanine on Signal Sequence
  • GenBank ID
  • Promoter T7 Promoter
  • Tags / Fusion Proteins
    • N.meningitidis signal sequence (N terminal on insert)
    • 10x Histidine Tag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NmMetQ C20A was a gift from Douglas Rees (Addgene plasmid # 172112 ; http://n2t.net/addgene:172112 ; RRID:Addgene_172112)

  • For your References section:

    Characterization of the ABC methionine transporter from Neisseria meningitidis reveals that lipidated MetQ is required for interaction. Sharaf NG, Shahgholi M, Kim E, Lai JY, VanderVelde DG, Lee AT, Rees DC. Elife. 2021 Aug 19;10. pii: 69742. doi: 10.7554/eLife.69742. 10.7554/eLife.69742 PubMed 34409939
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