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Addgene

pEGFP-eDHFR-MCS
(Plasmid #172108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4710
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-eDHFR-20aa
  • Species
    E. coli
  • Insert Size (bp)
    555
  • Entrez Gene
    folA (a.k.a. b0048, ECK0049, tmr, tmrA)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-eDHFR-MCS was a gift from Shinya Tsukiji (Addgene plasmid # 172108 ; http://n2t.net/addgene:172108 ; RRID:Addgene_172108)

  • For your References section:

    Synthetic self-localizing ligands that control the spatial location of proteins in living cells. Ishida M, Watanabe H, Takigawa K, Kurishita Y, Oki C, Nakamura A, Hamachi I, Tsukiji S. J Am Chem Soc. 2013 Aug 28;135(34):12684-9. doi: 10.1021/ja4046907. Epub 2013 Aug 14. 10.1021/ja4046907 PubMed 23941503
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