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pCSIIpuro-mNG-eDHFR(69K6)-RasGEF
(Plasmid #172104)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172104 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSII-EF-MCS
  • Backbone manufacturer
    Dr. Hiroyuki Miyoshi (RIKEN, Japan)
  • Backbone size w/o insert (bp) 10309
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2144
  • Entrez Gene
    RASGRF1 (a.k.a. CDC25, CDC25L, GNRP, GRF1, GRF55, H-GRF55, PP13187, ras-GRF1)
  • Promoter EF1-alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSIIpuro-mNG-eDHFR(69K6)-RasGEF was a gift from Shinya Tsukiji (Addgene plasmid # 172104 ; http://n2t.net/addgene:172104 ; RRID:Addgene_172104)

  • For your References section:

    A chemogenetic platform for controlling plasma membrane signaling and synthetic signal oscillation. Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, Atsuta-Tsunoda K, Aoki K, Tsukiji S. Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13. 10.1016/j.chembiol.2022.06.005 PubMed 35835118
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