pPBpuro-mNG-eDHFR(69K6)-p85iSH2
(Plasmid
#172103)
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PurposeMammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172103 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPB
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Backbone manufacturerDr. Allan Bradley (Wellcome Sanger Institute)
- Backbone size w/o insert (bp) 6910
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)JM109
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemNeonGreen-eDHFR(69K6)-p85iSH2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1962
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Entrez GenePik3r2 (a.k.a. p85beta)
- Promoter CAG
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPBpuro-mNG-eDHFR(69K6)-p85iSH2 was a gift from Shinya Tsukiji (Addgene plasmid # 172103 ; http://n2t.net/addgene:172103 ; RRID:Addgene_172103) -
For your References section:
A chemogenetic platform for controlling plasma membrane signaling and synthetic signal oscillation. Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, Atsuta-Tsunoda K, Aoki K, Tsukiji S. Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13. 10.1016/j.chembiol.2022.06.005 PubMed 35835118