pChd4EosHaloTAF_Puro
(Plasmid
#172099)
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PurposeTargeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 8790
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Vector typeMouse Targeting
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namechromodomain helicase DNA binding protein 4
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Alt nameCHD4
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SpeciesM. musculus (mouse), Synthetic
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Insert Size (bp)6360
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GenBank ID
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Entrez GeneChd4 (a.k.a. 9530019N15Rik, D6Ertd380e, Mi-2beta, mKIAA4075)
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Tags
/ Fusion Proteins
- Eos (C terminal on insert)
- Halo (C terminal on insert)
- TEV (C terminal on insert)
- Avi (C terminal on insert)
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pChd4EosHaloTAF_Puro was a gift from Brian Hendrich (Addgene plasmid # 172099 ; http://n2t.net/addgene:172099 ; RRID:Addgene_172099)