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Addgene

pChd4EosHaloTAF_Puro
(Plasmid #172099)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172099 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript II KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 8790
  • Vector type
    Mouse Targeting
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    chromodomain helicase DNA binding protein 4
  • Alt name
    CHD4
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    6360
  • GenBank ID
  • Entrez Gene
    Chd4 (a.k.a. 9530019N15Rik, D6Ertd380e, Mi-2beta, mKIAA4075)
  • Tags / Fusion Proteins
    • Eos (C terminal on insert)
    • Halo (C terminal on insert)
    • TEV (C terminal on insert)
    • Avi (C terminal on insert)
    • 3xFLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pChd4EosHaloTAF_Puro was a gift from Brian Hendrich (Addgene plasmid # 172099 ; http://n2t.net/addgene:172099 ; RRID:Addgene_172099)