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pET28a-Gluc-EL222-his
(Plasmid #172097)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172097 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5274
  • Total vector size (bp) 6507
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositor recommends using SHuffle T7 E. coli for correct folding of the protein during expression (due to multiple disulfide bonds)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gaussia Luciferase - EL222
  • Alt name
    Gluc-EL222
  • Species
    Synthetic
  • Insert Size (bp)
    1233
  • Mutation
    Gluc mutations - M43L, M110L
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-Gluc-EL222-his was a gift from Avi Schroeder (Addgene plasmid # 172097 ; http://n2t.net/addgene:172097 ; RRID:Addgene_172097)

  • For your References section:

    Synthetic cells with self-activating optogenetic proteins communicate with natural cells. Adir O, Albalak MR, Abel R, Weiss LE, Chen G, Gruber A, Staufer O, Kurman Y, Kaminer I, Shklover J, Shainsky-Roitman J, Platzman I, Gepstein L, Shechtman Y, Horwitz BA, Schroeder A. Nat Commun. 2022 Apr 28;13(1):2328. doi: 10.1038/s41467-022-29871-8. 10.1038/s41467-022-29871-8 PubMed 35484097
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