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6xHis-CPL2/pET28a
(Plasmid #172095)

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Item Catalog # Description Quantity Price (USD)
Plasmid 172095 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 7676
  • Modifications to backbone
    NA
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CPL2
  • Alt name
    CER11
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2313
  • GenBank ID
    NM_120205
  • Entrez Gene
    CPL2 (a.k.a. AT5G01270, ATCPL2, F7J8.250, F7J8_250, carboxyl-terminal domain (ctd) phosphatase-like 2)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6xHis-CPL2/pET28a was a gift from George Haughn (Addgene plasmid # 172095 ; http://n2t.net/addgene:172095 ; RRID:Addgene_172095)

  • For your References section:

    ECERIFERUM11/C-TERMINAL DOMAIN PHOSPHATASE-LIKE2 Affects Secretory Trafficking. Shi L, Dean GH, Zheng H, Meents MJ, Haslam TM, Haughn GW, Kunst L. Plant Physiol. 2019 Nov;181(3):901-915. doi: 10.1104/pp.19.00722. Epub 2019 Sep 4. 10.1104/pp.19.00722 PubMed 31484679
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