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Addgene

VHACg/pDONR221
(Plasmid #172094)

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Item Catalog # Description Quantity Price (USD)
Plasmid 172094 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4762
  • Total vector size (bp) 7926
  • Modifications to backbone
    none
  • Vector type
    Gateway Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VHA-C genomic region including 530bp upstream of the ATG, exons, introns, and no stop codon
  • Alt name
    DET3
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    3225
  • GenBank ID
    NC_003075.7
  • Entrez Gene
    DET3 (a.k.a. AT1G12840, ARABIDOPSIS THALIANA VACUOLAR ATP SYNTHASE SUBUNIT C, ATVHA-C, DE-ETIOLATED 3, F13K23.9, F13K23_9, VACUOLAR ATP SYNTHASE SUBUNIT C)
  • Promoter 530bp upstream of VHA-C ATG

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VHACg/pDONR221 was a gift from George Haughn (Addgene plasmid # 172094 ; http://n2t.net/addgene:172094 ; RRID:Addgene_172094)

  • For your References section:

    ECERIFERUM11/C-TERMINAL DOMAIN PHOSPHATASE-LIKE2 Affects Secretory Trafficking. Shi L, Dean GH, Zheng H, Meents MJ, Haslam TM, Haughn GW, Kunst L. Plant Physiol. 2019 Nov;181(3):901-915. doi: 10.1104/pp.19.00722. Epub 2019 Sep 4. 10.1104/pp.19.00722 PubMed 31484679
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