CPL2g/pDONR221
(Plasmid
#172093)
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PurposeGenomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172093 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4761
- Total vector size (bp) 11531
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Vector typeGateway Entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCPL2
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Alt nameCER11
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SpeciesA. thaliana (mustard weed)
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Entrez GeneCPL2 (a.k.a. AT5G01270, ATCPL2, F7J8.250, F7J8_250, carboxyl-terminal domain (ctd) phosphatase-like 2)
- Promoter CPL2
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13For
- 3′ sequencing primer M13Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note that the location of the stop codon based on TAIR gene model AT5G01270.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CPL2g/pDONR221 was a gift from George Haughn (Addgene plasmid # 172093 ; http://n2t.net/addgene:172093 ; RRID:Addgene_172093) -
For your References section:
ECERIFERUM11/C-TERMINAL DOMAIN PHOSPHATASE-LIKE2 Affects Secretory Trafficking. Shi L, Dean GH, Zheng H, Meents MJ, Haslam TM, Haughn GW, Kunst L. Plant Physiol. 2019 Nov;181(3):901-915. doi: 10.1104/pp.19.00722. Epub 2019 Sep 4. 10.1104/pp.19.00722 PubMed 31484679