EIF2B3-R312Q
(Plasmid #172085)

• Purpose: In vitro transcription for rescue experiments
• Depositing Lab: Cheol-Hee Kim
• Publication: Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342. doi: 10.1093/hmg/ddab033.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EIF2B3
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• Mutation: c.935G>A
• Entrez Gene: EIF2B3 (a.k.a. EIF-2B, EIF2Bgamma)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry