pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)
(Plasmid
#172065)
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PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172065 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepBS-KS-attB1-2-PT-SA-SD-1
- Backbone size w/o insert (bp) 3394
- Total vector size (bp) 4060
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTagBFP2(11x7)
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SpeciesD. melanogaster (fly)
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Insert Size (bp)657
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer GTAAAACGACGGCCAG
- 3′ sequencing primer GGAAACAGCTATGACCATG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7) was a gift from Daichi Kamiyama (Addgene plasmid # 172065 ; http://n2t.net/addgene:172065 ; RRID:Addgene_172065) -
For your References section:
Cell-type-specific, multicolor labeling of endogenous proteins with split fluorescent protein tags in Drosophila. Kamiyama R, Banzai K, Liu P, Marar A, Tamura R, Jiang F, Fitch MA, Xie J, Kamiyama D. Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). pii: 2024690118. doi: 10.1073/pnas.2024690118. 10.1073/pnas.2024690118 PubMed 34074768