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Addgene

pACUH-GFP1-10 sec
(Plasmid #172062)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172062 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACUH
  • Backbone manufacturer
    Yuh-Nung Jan
  • Backbone size w/o insert (bp) 9336
  • Total vector size (bp) 10049
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP1-10 sec
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    708

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TAACCAGCAACCAAGTAAAT
  • 3′ sequencing primer GCTTTAAATCTCTGTAGGTAGTTTGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACUH-GFP1-10 sec was a gift from Daichi Kamiyama (Addgene plasmid # 172062 ; http://n2t.net/addgene:172062 ; RRID:Addgene_172062)

  • For your References section:

    Cell-type-specific, multicolor labeling of endogenous proteins with split fluorescent protein tags in Drosophila. Kamiyama R, Banzai K, Liu P, Marar A, Tamura R, Jiang F, Fitch MA, Xie J, Kamiyama D. Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). pii: 2024690118. doi: 10.1073/pnas.2024690118. 10.1073/pnas.2024690118 PubMed 34074768
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