M64 pGEX4T-1-TEV-human beta cat domain N(1-137)
(Plasmid
#17205)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17205 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T-1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namebeta catenin
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Alt nameb-catenin
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SpeciesH. sapiens (human)
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Insert Size (bp)411
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Mutationamino-terminal residues 1-137
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Entrez GeneCTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site SalI / XhoI (destroyed during cloning)
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Description: This is an amino terminal human beta catenin residues 1-137 downstream of the GST fusion. Cloned as a BamHI-SalI fragment into BamHI and XhoI sites.
Reference: Made by Ken-Ichi Takemaru.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M64 pGEX4T-1-TEV-human beta cat domain N(1-137) was a gift from Randall Moon (Addgene plasmid # 17205 ; http://n2t.net/addgene:17205 ; RRID:Addgene_17205)