M63 pPET28a-TEV-human beta cat domain C(666-781)
(Plasmid
#17204)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-28 a
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namebeta catenin
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Alt nameb-catenin
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SpeciesH. sapiens (human)
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Insert Size (bp)345
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Mutationcarboxy-terminal residues 666-781
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Entrez GeneCTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
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Tag
/ Fusion Protein
- HIS (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI / SalI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Description: This is an amino terminal HIS tag, then TEV protease site, then human beta catenin carboxy terminal residues 666-781, as a BamHI-XhoI fragment cloned into BamHI and SalI sites.
Reference: Made by Ken-Ichi Takemaru.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M63 pPET28a-TEV-human beta cat domain C(666-781) was a gift from Randall Moon (Addgene plasmid # 17204 ; http://n2t.net/addgene:17204 ; RRID:Addgene_17204)