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M60 pPET28a-Xenopus beta catenin domains NR7
(Plasmid #17201)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17201 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28 a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    beta catenin
  • Alt name
    b-catenin
  • Species
    X. laevis (frog)
  • Mutation
    domains NR7
  • Entrez Gene
    ctnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
  • Tag / Fusion Protein
    • HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: This is an amino terminal HIS tag, then beta catenin domains NR7, subcloned into BamH1 and Sal1 sites.

Reference: Made by Ken-Ichi Takemaru.

Contains S33A, S37A, T41A, and S45A mutations, which inhibit phosphorylation and results in protein stabilization.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M60 pPET28a-Xenopus beta catenin domains NR7 was a gift from Randall Moon (Addgene plasmid # 17201 ; http://n2t.net/addgene:17201 ; RRID:Addgene_17201)
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