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pBMN-FKBP DD(L106P)-UbK0G75C-sfGFP
(Plasmid #172001)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172001 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBMN
  • Total vector size (bp) 7547
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FKBP DD (L106P)-UbK0G75C
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    567
  • Mutation
    change all lysin residues of ubiquitin to Arg, G75 was substituted to Cys
  • Tag / Fusion Protein
    • sfGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NsiI (unknown if destroyed)
  • 5′ sequencing primer pBMN 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN-FKBP DD(L106P)-UbK0G75C-sfGFP was a gift from Thomas Wandless (Addgene plasmid # 172001 ; http://n2t.net/addgene:172001 ; RRID:Addgene_172001)

  • For your References section:

    A Method for Conditional Regulation of Protein Stability in Native or Near-Native Form. Miyamae Y, Chen LC, Utsugi Y, Farrants H, Wandless TJ. Cell Chem Biol. 2020 Dec 17;27(12):1573-1581.e3. doi: 10.1016/j.chembiol.2020.09.004. Epub 2020 Oct 1. 10.1016/j.chembiol.2020.09.004 PubMed 33007216
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