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M49 GST-TEV-Chibby-C
(Plasmid #17197)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17197 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Chibby
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    190
  • Mutation
    amino acids 64-126
  • Entrez Gene
    CBY1 (a.k.a. C22orf2, CBY, Chibby1, HS508I15A, PGEA1, PIGEA-14, PIGEA14, arb1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pGEX 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: The C terminal half (amino acids 64-126) of human Chibby was amplified by PCR and subcloned into EcoR1 and Xho1 sites of pGEX-4T-1. A TEV protease site was included in the 5' primer. Insert contains 3'UTR of Cby.

Reference: K. Takemaru et al., Nature 422: 905 (2003)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M49 GST-TEV-Chibby-C was a gift from Randall Moon (Addgene plasmid # 17197 ; http://n2t.net/addgene:17197 ; RRID:Addgene_17197)

  • For your References section:

    Chibby, a nuclear beta-catenin-associated antagonist of the Wnt/Wingless pathway. Takemaru K, Yamaguchi S, Lee YS, Zhang Y, Carthew RW, Moon RT. Nature. 2003 Apr 24. 422(6934):905-9. 10.1038/nature01570 PubMed 12712206
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