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pcDNA3.1-TUBG2
(Plasmid #171966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    thermofisher
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6784
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TUBG2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1356
  • Mutation
    Removal of the internal TUBG2-EcoRI site by changing the nucleotide from a to g at position 117
  • GenBank ID
    NM_016437.3
  • Entrez Gene
    TUBG2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mCherry tagged TUBG2/pReceiver-M56 was purchased from GeneCopeia, Rockville, USA.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The internal EcoRI was removed from the GeneCopeia mCherry tagged TUBG2/pReceiver-M56 plasmid and TUBG2 was sub-cloned into pcDNA3.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-TUBG2 was a gift from Maria Alvarado-Kristensson (Addgene plasmid # 171966 ; http://n2t.net/addgene:171966 ; RRID:Addgene_171966)

  • For your References section:

    The gamma-tubulin meshwork assists in the recruitment of PCNA to chromatin in mammalian cells. Corvaisier M, Zhou J, Malycheva D, Cornella N, Chioureas D, Gustafsson NMS, Rossello CA, Ayora S, Li T, Ekstrom-Holka K, Jirstrom K, Lindstrom L, Alvarado-Kristensson M. Commun Biol. 2021 Jun 22;4(1):767. doi: 10.1038/s42003-021-02280-1. 10.1038/s42003-021-02280-1 PubMed 34158617
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