M48 GST-TEV-Chibby-N
(Plasmid
#17196)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17196 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4T-1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameChibby
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SpeciesH. sapiens (human)
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Insert Size (bp)190
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Mutationamino acids 1-63
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Entrez GeneCBY1 (a.k.a. C22orf2, CBY, Chibby1, HS508I15A, PGEA1, PIGEA-14, PIGEA14, arb1)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Description: The N terminal half (amino acids 1-63) of human Chibby was amplified by PCR and subcloned into EcoR1 and Xho1 sites of pGEX-4T-1. A TEV protease site was included in the 5' primer.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M48 GST-TEV-Chibby-N was a gift from Randall Moon (Addgene plasmid # 17196 ; http://n2t.net/addgene:17196 ; RRID:Addgene_17196) -
For your References section:
Chibby, a nuclear beta-catenin-associated antagonist of the Wnt/Wingless pathway. Takemaru K, Yamaguchi S, Lee YS, Zhang Y, Carthew RW, Moon RT. Nature. 2003 Apr 24. 422(6934):905-9. 10.1038/nature01570 PubMed 12712206