pHIS8-3_DL121
(Plasmid #171953)

• Purpose: Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS
• Depositing Lab: Kimberly Reynolds
• Publication: McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi: 10.7554/eLife.68346.

Backbone

• Vector backbone: phis8-3
• Backbone size w/o insert (bp): 5206
• Total vector size (bp): 6204
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Outgrowth to OD 0.7 at 30C, induce with IPTG, grow O.N 18C.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fusion of ecDHFR and asLOV2 at position 121 of DHFR
• Alt name: DL121
• Species: Escherichia coli
• Insert Size (bp): 888
• Promoter: T7
• Tag / Fusion Protein:
  • 8-His tag (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Supplemental Documents:
  • pHIS8-3_DL121.gb
• A portion of this plasmid was derived from a plasmid made by: Kimberly Reynolds

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Designed and introduced as A120-NoJ in PMID: 18927392, Further described as DL121 in PMID: 22196731