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pHIS8-3_DL121
(Plasmid #171953)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171953 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    phis8-3
  • Backbone size w/o insert (bp) 5206
  • Total vector size (bp) 6204
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Outgrowth to OD 0.7 at 30C, induce with IPTG, grow O.N 18C.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fusion of ecDHFR and asLOV2 at position 121 of DHFR
  • Alt name
    DL121
  • Species
    Escherichia coli
  • Insert Size (bp)
    888
  • Promoter T7
  • Tag / Fusion Protein
    • 8-His tag (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Kimberly Reynolds

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Designed and introduced as A120-NoJ in PMID: 18927392, Further described as DL121 in PMID: 22196731

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIS8-3_DL121 was a gift from Kimberly Reynolds (Addgene plasmid # 171953 ; http://n2t.net/addgene:171953 ; RRID:Addgene_171953)

  • For your References section:

    Structurally distributed surface sites tune allosteric regulation. McCormick JW, Russo MA, Thompson S, Blevins A, Reynolds KA. Elife. 2021 Jun 16;10. pii: 68346. doi: 10.7554/eLife.68346. 10.7554/eLife.68346 PubMed 34132193
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