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M47 MBP-TEV-Chibby
(Plasmid #17195)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17195 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAL-c2E
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Chibby
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Entrez Gene
    CBY1 (a.k.a. C22orf2, CBY, Chibby1, HS508I15A, PGEA1, PIGEA-14, PIGEA14, arb1)
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII / BamHI (destroyed during cloning)
  • 3′ cloning site XhoI / SalI (destroyed during cloning)
  • 5′ sequencing primer M13 forward 20
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: The full coding sequence of human Chibby, including 3' UTR was amplified by PCR, digested with BglII and Xho1, and subcloned into the BamH1 and Sal1 sites of pMAL-c2E. The TEV protease site was included in the 5'primer. Insert size ~1kb.

Reference: K. Takemaru et al., Nature 422: 905 (2003)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M47 MBP-TEV-Chibby was a gift from Randall Moon (Addgene plasmid # 17195 ; http://n2t.net/addgene:17195 ; RRID:Addgene_17195)

  • For your References section:

    Chibby, a nuclear beta-catenin-associated antagonist of the Wnt/Wingless pathway. Takemaru K, Yamaguchi S, Lee YS, Zhang Y, Carthew RW, Moon RT. Nature. 2003 Apr 24. 422(6934):905-9. 10.1038/nature01570 PubMed 12712206
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